| BLVRB

Biliverdin reductase B is a protein that is encoded by the BLVRB gene. The BLVRB gene was localized to chromosome 19, the specific region being 19q13.13 to q13.2; this was done using fluorescence in situ hybridization. The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B . From a functional standpoint, it has been hypothesized that BLRVB is identical to flavin reductase (FR), an enzyme that catalyzes the NADPH-dependent reduction of FMN and Methylene Blue and, in the presence of redox couplers, the reduction of methaemoglobin.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.02	1.27	0.8	0.49	0.34	0.25	0.19	0.07
Corrected OD	1.95	1.2	0.73	0.42	0.27	0.18	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human BLVRB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human BLVRB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
	Average (%)	98	108